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Gastrin-17 (G-17)

產(chǎn)品參數(shù)

Anti-G-17 Antibody (Datasheet)

Source Mouse monoclonal antibody, cultured in vitro
Platforms Immunofluorescence, Chemiluminescence, and Colloidal gold
Catalog number K55a9 K57v2
Applications Capture Detection
Epitopes Native G-17 protein
Buffer 1x PBS
Purity Purity>98%,purified by Protein A/G chromatography
Storage instructions Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Product Information

Antibody Product Information

Antibody Evaluation (Immunofluorescence-based)

Immunofluorescence-Based test strip was prepared with K55a9-K57v2 matched antibody pairs (30 Biohit calibrators, Concentration: 1-50 pmol/L, R2 >96%)
Fig. 1 Coincidence rate of Immunofluorescence-based sample

Fig. 1 Coincidence rate of Immunofluorescence-based sample

Accuracy
The quality control samples with concentration of 3 pmol/L-7 pmol/L and 15 pmol/L-50 pmol/L respectively were required to repeat test three times. B= (M-T) / T×100% (B: Relative Standard Deviation, M: Average, T: Concentration)
Sample(pmol/L) Test 1 Test 2 Test 3 Average(pmol/L) RSD Standard
Batch 1 5.21 5.38 5.54 5.45 5.46 4.73% ±15%
20.55 18.55 21.47 23.47 21.16 2.98%
Batch 2 5.21 5.62 5.5 5.14 5.42 4.03%
20.55 20.15 19.4 20.62 20.06 -2.40%
Batch 3 5.21 5.29 5.54 5.69 5.51 5.69%
20.55 21.16 20.86 22.48 21.50 4.62%
Repeatability
The quality control samples with concentration of 3 pmol/L-7 pmol/L and 15 pmol/L-25 pmol/L respectively were required to repeat test over 10 times. CV= (S /average) ×100% (CV < 15% as indicated in the following table)
Low Value
(5.21pmol/L)
High Value
(20.55pmol/L)
Test 1 5.83 22.69
Test 2 5.08 19.45
Test 3 5.49 19.98
Test 4 5.28 19.06
Test 5 5.83 22.85
Test 6 5.82 22.69
Test 7 5.91 22.92
Test 8 5.24 20.97
Test 9 5.22 19.07
Test 10 5.81 18.49
Average (pmol/L) 5.55 20.82
SD 0.32 1.82
CV 5.79% 8.73%
Standard ±15%
Clinical Significance

Description

Human progastrin consists of 80 amino acids and is cleaved to two biologically active forms, gastrin-34 and gastrin-17. Over 90% of the gastrin present in the antral mucosa is the G17 form. The secretion of G-17 is mainly affected by intragastric pH value, G cell number and diet. It is a sensitive indicator of the secretory function of gastric antrum and plays important roles in the diagnosis and screening of atrophic gastritis and gastric cancer.
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